Main page
Education

Biochemistry - Practice

Data

Official data in SubjectManager for the following academic year: 2023-2024

Course director

Number of hours/semester

lectures: 0 hours

practices: 28 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBA-108-G
  • 2 kredit
  • Biotechnology MSc
  • Basic modul
  • autumn
Prerequisites:

OBA-108-E parallel

Course headcount limitations

min. 5 – max. 20

Available as Campus course for . Campus-karok: ÁOK

Topic

The course helps to evolve good practical skills. It gives an overview on the techniques that are used in the basic biochemical-, biomedical research, both in the industrial area as well as in clinical research. The main focus is on solving of different scientific problems and through that a solution orientated education.

Lectures

Practices

  • 1. Solutions
  • 2. Solutions
  • 3. Preparation of buffer solution
  • 4. Preparation of buffer solution
  • 5. Protein function
  • 6. Basics of enzyme catalysis
  • 7. Measuring the proteint contant of different solutions
  • 8. Biuret reaction
  • 9. Oral presentations
  • 10. Oral presentations
  • 11. Enzyme activity measurement
  • 12. Inorganic phosphate demand of glycolysis
  • 13. Oral presentations
  • 14. Oral presentations
  • 15. Molecular recognition
  • 16. Affinity chromatography
  • 17. Apoptosis, necrosis
  • 18. Techniques to describe apoptotical processes or necrosis
  • 19. PAGE, Western blot
  • 20. PAGE, Western blot
  • 21. Antibody-Based techniques in biochemistry
  • 22. Antibody-Based techniques in biochemistry
  • 23. Working with cells
  • 24. Working with cells
  • 25. Bioanalytical teachniques
  • 26. Bioanalytical teachniques
  • 27. Consultation
  • 28. Exam

Seminars

Reading material

Obligatory literature

Albert L. Lehninger - David L. Nelson - Michael M.Cox: Principles of Biochemistry, Worth Publichers, Inc.

Literature developed by the Department

Teams: OBA-108-G-2023241-MEA-A108-G-B1
http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Notes

Teams: OBA-108-G-2023241-MEA-A108-G-B1
http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Recommended literature

Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer: Biochemistry
Thomas M. Devlin: Textbook of Biochemistry. With Clinical Correlations, John Wiley & Sons, New York

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Oral presentations should be kept by every student on the 5th or 7th week from an optional topic, in the from of a ppt presentation. The theme of the presentation should have a biochemical connection, and should be adjusted with the subject leader.
A written exam is going to be written in the 14th week of the semester that screens the basic methodological knowledge, and the capability of solving scientific problems. In case of unsuccesful exam, it can be retaken in the first two weeks of the exam periode.

Making up for missed classes

There is no possibility to retake the practice.

Exam topics/questions

Teams: OBA-108-G-2023241-MEA-A108-G-B1

Examiners

Instructor / tutor of practices and seminars

  • Dr. Juhász Kata
  • Dr. Takátsy Anikó
  • Nagyné Dr. Kiss Gyöngyi
Rólunk